The impact of human dominion is widely documented, including driving plants toward extinction and facilitating range expansions in others (aka. plant invasions). Most of the world’s rivers have been altered for human usage, whether that be for drinking water, flood mitigation, agriculture, or a mix of these. A consequence of modified flow regimes is an increase in plant invasions as this typically changes native landscapes in ways that provide invading plants with a competitive advantage. My research investigates seed bank assemblages in the northern Murray-Darling Basin, a region subjected to augmented river flows. Instream, riparian and floodplain seed bank types were collected from across the region and put through germination trials. I found distinctive patterns in seed bank assemblages for rivers subjected to different levels of natural versus managed flow. These patterns reveal that many exotic species are arriving in new landscapes, many of which that aren’t currently being expressed in the extant communities. This research has implications for environmental management, particularly regarding potential for future species invasions under climate change scenarios.