Many aquatic invertebrates have adaptations to survive when ephemeral water bodies dry. In rock pools of the Sonoran Desert, Odontomyia sp. (Stratiomyidae) larvae undergo some form of dormancy to survive drying and continue their lives when water returns. However, we do not know any details of how they do so, nor do we know how many drying cycles they can survive with this adaptation. This is a concern because the increasing frequency and duration of drying events due to climate change may push these fly larvae beyond their physiological limits. To examine these issues, we conducted a series of lab experiments to test: i) the number of drying cycles the larvae can survive, ii) how drying frequency affects body mass and survival, iii) how drying extent affects body mass and survival, and iv) how temperature affects survival through dormancy. Overall, survival in the lab was lower than expected. Some Odontomyia larvae can survive at least two drying cycles, though survival decreases with each drying event. There was no consistent effect of drying frequency on body mass. Enhanced moisture levels in the sediment where dormant larvae are present did not seem to affect body mass or survival, though replication was low due to the limited number of surviving larvae. In terms of temperature, one larva was revived after drying at 40°C for 17 days, but all other larvae in the temperature trials perished. Although these results suggest Odontomyia are fairly resistant to drying, more data is needed about how body mass, pupation, and reproductive success change with drying events. The more we understand about drying adaptations of rock pool aquatic invertebrates, the more successful conservation plans will be for aquatic biota in a changing climate.