The effects of river regulation on wetlands and floodplains are recognised as a key threat to amphibian communities which continue to face global declines. Frogs are an important component of the wetland food chain due to both an aquatic and terrestrial life phase and are considered good indicators of wetland health. Prior work on amphibians in regulated river catchments has indicated that wetland inundation from high river flows influences breeding in some frog species more than others. Our aim was to identify flow metrics (i.e., magnitude, timing, and duration of wetland inundation) linked to breeding and recruitment success in wetland-dependant frogs. We conducted repeat annual spring-summer surveys of frog communities between 2015 and 2020 at 29 sites across two important wetland regions in the northern Murray-Darling Basin, the Gwydir Wetlands and Macquarie Marshes. We focused our analyses on species found to be flow-dependent, that is species which breed in response to wetland inundation, regardless of other factors such as rainfall. Using Random Forest analyses, we investigated the importance of 22 explanatory variables on breeding activity, including several hydrological measures (preceding stream flow volume, extent of inundation and duration of inundation) and other key variables with known or suspected influences on breeding by these species. Of all variables considered and regardless of catchment, we found that timing and extent of inundation were the most important drivers of calling activity (indicating breeding attempts), while the volume of preceding river flows, as a measure of inundation duration, was most important for breeding success, based on abundance of frogs that had recently completed metamorphosis. Based on our results, we developed principles to guide environmental water management aimed at influencing frog population persistence at regulated wetlands and highlight key considerations for evaluating those management actions.