Per- and polyfluoroalkyl substances (PFAS) are synthetic fluorinated organic substances of emerging concerns, due to their adverse health effects on humans and high environmental persistence. The special properties of PFAS, such as repelling stains and water, and strong heat resistance give them numerous industrial uses, which make them ubiquitous. The PFAS are used, for example, in cook- and baking-ware coatings, paints, varnishes, fire-fighting foams, floor covering such as carpets and floor polishes. They are also used in paper and packaging, wire and cable insulation, gaskets and hoses, and waterproof clothes. Studies on PFAS have focused on identifying and cataloging different forms of PFAS, creating detection tools, and quantifying them in various matrices, and uncovering their effects on human health. Nevertheless, very little has been done around understanding better the fate of PFAS in aquatic biota. The present project examines differences in bioaccumulation of various PFAS among benthic macroinvertebrates of varied trophic functions, reflecting different uptake pathways. Given macroinvertebrates are among organisms occupying the food-chain entry level, this project is intended to enable further predictions to gauge biomagnification and cascading effects of PFAS in freshwater ecosystems, as well as potential threats to public health. Macroinvertebrates and water samples were collected in four sites along the Mousam River, Maine, and supplemented with the data collected in a highly contaminated pond in Fairfield, Maine. Additionally, novel integrative passive samplers were deployed at each site for six-week duration to derive time-weighted averaged water concentrations of PFAS. Preliminary results will be available by June 2023 and discussed in the conference.